A multivariate cell-based liquid biopsy for lung nodule risk stratification: Analytical validation and early clinical evaluation
This study establishes the analytical reproducibility and biological relevance of the LC-iCAP. While clinical validation is preliminary, the results support the assay's potential utility in lung nodule management. The study introduces a new paradigm of using scalable and cost-effective cell-based biosensor assays for liquid biopsies. With a multivariate readout, the platform is amenable to precision medicine applications such as multi-cancer early detection.
Authors
- Marcello D’Ascenzo (ORCID: https://orcid.org/0000-0003-0073-412X)
- Fergal J. Duffy (ORCID: https://orcid.org/0000-0002-4675-0937)
- G. Adam Whitney (ORCID: https://orcid.org/0000-0003-0771-3532)
- Anil Vachani (ORCID: https://orcid.org/0000-0002-3871-8697)
- Leslie R. Miller (ORCID: https://orcid.org/0000-0002-2555-2771)
- Stephen A. Deppen (ORCID: https://orcid.org/0000-0002-3661-662X)
- Jason D. Berndt (ORCID: https://orcid.org/0000-0002-7388-6792)
- Pierre P. Massion (ORCID: https://orcid.org/0000-0003-0647-0559)
- Yijun Qi (ORCID: https://orcid.org/0000-0002-7100-1700)
- Robert J. Lipshutz
- Samuel A. Danziger (ORCID: https://orcid.org/0000-0002-5669-414X)
- John D. Aitchison (ORCID: https://orcid.org/0000-0001-5226-7709)
- Jennifer J. Smith (ORCID: https://orcid.org/0000-0001-5780-7209)
Institutions
- Vanderbilt University (US)
- University of Washington (US)
- Institute for Systems Biology (US)
- Pulmonary and Allergy Associates (US)
- PreCyte (United States) (US)
- University of Pennsylvania (US)
- Vanderbilt University Medical Center (US)
Publication Details
- Journal
- The Journal of Liquid Biopsy
- Published
- 2025-07-26
- DOI
- https://doi.org/10.1016/j.jlb.2025.100313
- Primary Topic
- Lung Cancer Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Vanderbilt University
- University of Pennsylvania
- National Institutes of Health
- National Institute on Aging
- National Cancer Institute
- National Institute of Environmental Health Sciences