Cytotoxic Effects of “25.2% Boscalid + 12.8% Pyraclostrobin” Fungicide

It is known that different pesticides used against domestic or agricultural pests have toxic effects. In this study, 25.2% boscalid and 12.8% pyraclostrobin were used in the test material. Kate A1 Russian wheat variety was used for Triticum growth inhibition tests. According to the Triticum root and stem growth test, the concentration value that halves the root and stem length is known as the "EC50 value" According to the test, the root length of the control group was 6.98 ± 0.65 cm and the length of the stem was 9.36 ± 0.71 cm. According to the Triticum test, the EC50 value of the fungicide was determined as 2500 ppm. The value that halves the stem length of the control group was determined as 1250 ppm. Some doses of this fungicide (625, 1250, 2500, 5000, and 10,000 ppm) were observed to inhibit root and stem growth, and these concentrations' results were statistically significant according to Dunnett's t-test. In the root mitotic index analysis, 5000 cells were counted for each concentration, and it was determined that all concentrations tested had negative effects on mitotic activity. It was observed that the concentration of 10,000 ppm was the most decreasing (0.53 ± 0.18 cm) mitotic index %. The recommended dose of the tested fungicide in the fight against agricultural pests is around 500 ppm, and this indicates that the fungicide will have limited cytogenetic effects. The mitotic index test indicated that 2500 and upper concentrations had toxic effects on the mitotic index. The most toxic effect was in the 10,000 ppm treatment. The MTT test showed that all concentrations had a cytotoxic effect on MDBK cells. But 625 ppm concentration in all treatment periods and 1250 ppm 24-h results were statistically no significant according to Dunnett's t-test.