Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage

Ensiling technique is becoming a common way to preserve fresh corn and grass. This study aimed at evaluating the silages’ quality with a new type of inoculants designed based on the microflora information of the native silages. A total of 33 silage samples were analyzed for their microbial community structure by high throughput sequencing. A total of 73 dominant strains were isolated with a clear goal. The corn was ensiled by dominant strains (Lactobacillus buchneri II JC1-5: Acetobacter pasteurianus DHL-8 = 4:3; 109 CFU/g) as inoculants. In a 15-day trial period, the aerobic stability of this inoculated silage was significantly higher than the control group. There was a positive correlation between lactic acid content and pH/mold number (R2 values of 0.64331 and 0.48584, respectively). Compared to the control group, ammonia nitrogen (NH3-N), water soluble carbohydrates (WSC), neutral detergent fiber (NDF), and acid detergent fiber (ADF) decreased by 57.7%, 12.5%, 1.4%, and 7.5%, respectively; crude protein (CP) increased by 1.1%. Our results indicate that the new type of inoculants can improve the quality of the silage.