In-vitro Antithrombotic potential of Ethanolic Active Fractions and Herbal Mixture containing Cordia macleodii Hook, Cissus repanda Vahl and Celosia argentea Linn

Objective: The existing intervention was intended to explore the relative examination of in- vitro thrombolytic potential of active ethanolic fraction each of Cordia macleodii bark (CMB), Cissus repanda root (CRR) and Celosia argentea root (CAR) separately and their combinations in different proportions. Methods: A model of in vitro thrombolysis was utilized to assess the thrombolytic impact of active fractions of chosen herbal medications dissolved in ethanol. The clot lysis effect of 100 μl of each herbal active fraction and equivalent proportions of herbal mixtures from CMB, CRR, and CAR were analyzed by comparing them with 100 μl streptokinase and 100 μl distilled water, used as positive and negative controls, respectively. Percentage of thrombolysis was assessed as a result of weight difference between microcentrifuge tubes before and after clot lysis. Results: Findings from experimental study reveals that streptokinase, positive control was able to dissolve 55.61 % of the clot and sterile distilled water, negative control shows 2.12 % clot lysis. The active ethanolic fraction each of CMB, CRR and CAR on an average were able to dissolve 22.63 %, 22.78 % and 26.63 % of the clot independently. Some combinations of these herbal active fractions in various proportions had been tried for thrombolytic activity, the most efficient combination of CMB, CRR and CAR fractions was found to be in (1:2:1) ratio respectively and was capable to split 51.49 % of clot. Conclusion: From the research findings it was determined that the mixture of active fractions of CMB, CRR and CAR in proportion of (1:2:1) correspondingly possessed significant thrombolytic potential compare to other proportions and individual active fractions of selected crude drugs used under study.