EFFECT OF LARIFAN ON MONOCYTES OF AGED C57BL/6 AND BALB/C MICE IN VITRO

Aim. This study aimed to evaluate the effect of Larifan on the metabolic profile of peripheral blood monocytes isolated from aged female BALB/c and C57Bl/6 mice in vitro. Methods. Peripheral blood was obtained from aged female BALB/c and C57BL/6 mice via facial vein puncture and exposed to Larifan treatment in vitro. Phagocytic activity, reactive oxygen species (ROS) generation, and surface marker expression were analyzed using flow cytometry. Results are expressed as median with interquartile range. Statistical significance was assessed using the Kruskal–Wallis test, with p-values less than 0.05 considered significant. Results. Larifan treatment led to a decrease in phagocytosis percentage in both BALB/c and C57BL/6 mice. The phagocytosis index slightly decreased in C57BL/6 mice while remaining unchanged in BALB/c. ROS production was higher in untreated C57BL/6 mice and decreased after treatment only in BALB/c. The number of CD80⁺ cells increased in C57BL/6 mice, while expression levels slightly decreased after the treatment. Larifan reduced the number of CD206⁺ cells in both strains and decreased CD206 expression in C57BL/6 mice only. Conclusions. Larifan exerted an anti-inflammatory effect in monocytes of aged BALB/c mice by reducing phagocytosis and ROS production. Treated cells from aged C57Bl/6 mice exhibited increased CD80 and reduced CD206 expression.