CATS: a bioinformatic tool for automated Cas9 nucleases activity comparison in clinically relevant contexts
By enabling the identification of overlapping PAMs and allele-specific targets, CATS supports the implementation of Cas9-based applications in both research and clinical settings. Its ability to incorporate genetic variants makes it particularly useful for designing therapeutic approaches that selectively target mutated alleles while sparing healthy ones. Ultimately, CATS contributes to the development of more effective and precise genetic therapies.
Authors
- Gastone Castellani (ORCID: https://orcid.org/0000-0003-4892-925X)
- Ettore Rocchi (ORCID: https://orcid.org/0000-0002-7612-2819)
- Antonio Carusillo (ORCID: https://orcid.org/0000-0001-5414-4390)
- Martina Tarozzi (ORCID: https://orcid.org/0000-0002-4240-0542)
- Federico Magnani (ORCID: https://orcid.org/0000-0003-4479-0916)
Institutions
- Istituti di Ricovero e Cura a Carattere Scientifico (IT)
- University of Bologna (IT)
Publication Details
- Journal
- Frontiers in Genome Editing
- Published
- 2025-07-24
- DOI
- https://doi.org/10.3389/fgeed.2025.1571023
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Associazione Italiana per la Ricerca sul Cancro
- Ministero dell'Università e della Ricerca