Comparing urine and stool gluten immunogenic peptides for detecting compliance to gluten-free diets
Detection of gluten immunogenic peptides (GIP) offers a promising objective biomarker for monitoring adherence to gluten-free diets. However, current methods require specialized labs and long processing times. By measuring GIP in stool, we identified children with Crohn's disease who were non-adherent despite standard assessments indicating otherwise. A point-of-care GIP kit demonstrated high sensitivity and specificity in detecting GIP in stool making it a potential alternative to the reference ELISA method. In contrast, GIP detection using urine kits was suboptimal. Our study supports the potential clinical utility of bedside point-of-care GIP kits that may improve treatment adherence and efficacy of gluten-free-diets.
Authors
- Konstantinos Gerasimidis (ORCID: https://orcid.org/0000-0001-9432-2200)
- Konstantinos Gkikas (ORCID: https://orcid.org/0000-0003-1581-6566)
- Vaios Svolos (ORCID: https://orcid.org/0000-0002-7785-4245)
- Maria Luı́sa Lima (ORCID: https://orcid.org/0000-0003-1171-2962)
- Laura Gianolio (ORCID: https://orcid.org/0009-0003-1189-9373)
- Caroline Kerbiriou (ORCID: https://orcid.org/0000-0002-7927-2740)
- Richard Kay Russell (ORCID: https://orcid.org/0000-0001-7398-4926)
- Richard Hansen (ORCID: https://orcid.org/0000-0002-3944-6646)
- M. Kavanagh
- Bernadette White (ORCID: https://orcid.org/0000-0001-7016-7429)
Institutions
- University of Thessaly (GR)
- University of Dundee (GB)
- University of Milan (IT)
- Royal Hospital for Children (GB)
- Glasgow Royal Infirmary (GB)
- e-Trikala (Greece) (GR)
- University of Glasgow (GB)
Publication Details
- Journal
- Pediatric Research
- Published
- 2025-07-24
- DOI
- https://doi.org/10.1038/s41390-025-04266-9
- Citations
- 2
- Primary Topic
- Celiac Disease Research and Management
- Type
- article
- Field-Weighted Citation Impact
- 3.62
Funders
- Leona M. and Harry B. Helmsley Charitable Trust
- Crohn's and Colitis Foundation
- University of Glasgow