Modulation of Pulmonary Fibrosis by Pulmonary Surfactant-Associated Phosphatidylethanolamine In Vitro and In Vivo
Pulmonary fibrosis (PF) is characterized by excessive collagen deposition and impaired lung function. Pulmonary surfactant may modulate fibroblast activity and offer therapeutic benefits. We developed a natural porcine pulmonary surfactant (NPPS) enriched with 1,2-dipalmitoyl-rac-glycero-3-phosphatidylethanolamine (PE) and evaluated its biophysical and biological properties. Biophysical analysis showed that PE improved surfactant performance by increasing surface pressure and stability. In vitro, NPPS-PE reduced collagen expression and induced apoptosis in normal human lung fibroblasts; in addition, it decreased proliferation in fibroblasts stimulated with TGF-β. In vivo, NPPS-PE improved gas exchange and significantly reduced collagen deposition in bleomycin-treated mice. These findings suggest that NPPS-PE may be a promising therapeutic strategy for fibrosing lung diseases.
Authors
- David A. Contreras‐Cruz (ORCID: https://orcid.org/0000-0002-9765-7097)
- María Guadalupe Hernández-Linares (ORCID: https://orcid.org/0000-0001-9413-4322)
- Beatriz Tlatelpa-Romero (ORCID: https://orcid.org/0000-0003-4080-2975)
- Gabriel Guerrero‐Luna (ORCID: https://orcid.org/0009-0004-0332-3785)
- Sinuhé Ruiz-Salgado
- Jesús Pérez‐Gil (ORCID: https://orcid.org/0000-0003-3587-7147)
- Criselda Mendoza-Milla (ORCID: https://orcid.org/0000-0003-0304-2313)
- Roberto Berra‐Romani (ORCID: https://orcid.org/0000-0003-4884-0925)
- Amaya Blanco-Rivero (ORCID: https://orcid.org/0000-0002-2954-6205)
- Bárbara Olmeda (ORCID: https://orcid.org/0000-0001-6004-1603)
- María Pérez-Fernández
- René de-la-Rosa Paredes
- Olga Cañadas (ORCID: https://orcid.org/0000-0002-1932-3796)
- Yair Romero (ORCID: https://orcid.org/0000-0002-8707-025X)
- Alonso Antonio Collantes-Gutiérrez
- Luis G. Vázquez-de-Lara Cisneros
- José Luis Martinez-Vaquero
Institutions
- Universidad Autónoma del Estado de Hidalgo (MX)
- Universidad Complutense de Madrid (ES)
- Universidad Autónoma de la Ciudad de México (MX)
- Hospital General de México (MX)
- Research Institute Hospital 12 de Octubre (ES)
- Instituto Nacional de Enfermedades Respiratorias (MX)
- Instituto Universitario Puebla (MX)
- Instituto de Química y Fisicoquímica Biológicas (AR)
- Benemérita Universidad Autónoma de Puebla (MX)
- Universidad Nacional Autónoma de México (MX)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2025-07-24
- DOI
- https://doi.org/10.3390/ijms26157132
- Primary Topic
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministerio de Ciencia e Innovación