Incorporating exon–exon junction reads enhances differential splicing detection
We demonstrate that incorporating exon-exon junction reads significantly improves the detection of differential splicing events. The proposed DEJU workflow offers increased statistical power and computational efficiency compared to widely used existing approaches, while effectively controlling the false discovery rate.
Authors
- Jane E. Visvader (ORCID: https://orcid.org/0000-0001-9173-6977)
- Yunshun Chen (ORCID: https://orcid.org/0000-0003-4911-5653)
- Michael J. G. Milevskiy (ORCID: https://orcid.org/0000-0002-7114-6272)
- Mai T. Pham (ORCID: https://orcid.org/0009-0001-6829-7053)
Institutions
- The University of Melbourne (AU)
- Walter and Eliza Hall Institute of Medical Research (AU)
Publication Details
- Journal
- BMC Bioinformatics
- Published
- 2025-07-24
- DOI
- https://doi.org/10.1186/s12859-025-06210-4
- Citations
- 1
- Primary Topic
- RNA Research and Splicing
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Victorian Cancer Agency
- Medical Research Council
- National Health and Medical Research Council