The prospects and challenge of pathogen diagnosisof lower respiratory tract infections in grassrootshospitals: The clinical practicality of targeted nextgenerationsequencing

1 virus, fungi, chlamydia and mycoplasma.In addition, for some immunocompromised patients, almost all bacteria or fungi can be considered potential pathogens in LRTIs 3 .Currently, conventional microbiological test methods (CMTs) including microscopy, pathogen culture and isolation, biochemical testing, enzyme-linked immunosorbent assay (ELSA), and polymerase chain reaction (PCR) testing, are applied to identify LRTIs pathogens.However, these methods have