EXPLORING THE THERAPEUTIC POTENTIAL OF SOUR CHERRY LEAF EXTRACT (PRUNUS CERASUS L.): A COMPREHENSIVE IN VITRO AND IN VIVO EVALUATION

Sour cherry leaves Prunus cerasus (PC) are a valuable natural source of bioactive compounds.This study assessed the antiinflammatory and anti-obesity properties of sour cherry aqueous extract.The phenolic profile was evaluated by highperformance liquid chromatography (RP-HPLC-PDA).The studied extract has a total phenolic content of 7.13 0.12 mg GAE per gram and exhibits a notable antioxidant effect determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays.Adding PC extract and orlistat (reference drug) to the high-fat diet (HFD) ameliorates the serum's lipid profile and reduces body gain by 9.84% and 15.61%, respectively.The histological sections demonstrate the effectiveness of the extract.The PC extract had no cytotoxic effect on RAW 264.7 cells and macrophages and inhibited nitrite production in a dose-dependent manner with an IC50 of nitric oxide inhibitory activity (NO) of 58.25 0.36 g/mL.The carrageenan-induced rat paw oedema model confirms the extract's anti-inflammatory potential in reducing paw thickness and improving oxidative stress parameters.In summary, sour cherry leaves could potentially be interesting for therapeutic use in controlling obesity and the treatment of inflammation. RezumatFrunzele de viin (Prunus cerasus -PC) reprezint o surs natural valoroas de compui bioactivi.n acest studiu au fost evaluate proprietile antiinflamatorii i antiobezitate ale extractului apos de viin.Profilul fenolic a fost analizat prin cromatografie lichid de nalt performan (RP-HPLC-PDA).Extractul analizat prezint un coninut total de compui fenolici de 7,13 0,12 mg echivaleni de acid galic (GAE) per gram i un efect antioxidant semnificativ, determinat prin testele DPPH i FRAP.Administrarea extractului de PC, precum i a orlistatului (medicament de referin), n cadrul unei diete bogate n grsimi (HFD), mbuntete profilul lipidic i scade rata de cretere n greutate cu 9,84%, respectiv 15,61%.Analiza histologic a evideniat eficacitatea extractului.De asemenea, extractul de PC nu a prezentat efect citotoxic asupra celulelor RAW 264.7 i a macrofagelor i a inhibat producia de nitrii ntr-o manier dependent de doz, cu o valoare IC pentru inhibarea oxidului nitric (NO) de 58,25 0,36 g/mL.Modelul de edem indus cu caragenan la obolani confirm potenialul antiinflamator al extractului, prin reducerea grosimii labei i mbuntirea parametrilor de stres oxidativ.n concluzie, frunzele de viin ar putea reprezenta o opiune prospectiv pentru utilizarea terapeutic n controlul obezitii i n tratamentul inflamaiei.