EFFECTS OF COPPER (II) THIOSEMICARBAZONES ON PRO-OXIDANT AND ANTIOXIDANT SYSTEM MARKERS IN IN VIVO ERYTHROCYTES

This study investigates the effects of copper (II) coordination compounds with thiosemicarbazones (Cu-CCTs) on oxidative stress markers and the antioxidant system in erythrocytes through an in vivo experiment.Methods: 120 rats (Rattus norvegicus albinus) were divided into 10 groups, further split into male and female subgroups.The control group received saline solution, while the other groups received 10 g/kg of Cu-CCTs subcutaneously for 30 days.Results: Cu-CCTs induced reactive oxygen species (ROS) formation and stimulated antioxidant defences, as evidenced by changes in malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and glutathione-related enzymes.Additionally, partial modulation of cell growth and apoptosis was inferred through the observed oxidative/antioxidative shifts -though further targeted assays are needed to confirm these mechanisms.Conclusions: Cu-CCTs exert a dual effect, elevating some pro-oxidant markers while simultaneously enhancing antioxidant responses, suggesting potential therapeutic relevance in conditions involving oxidative stress. RezumatAcest studiu investigheaz efectele compuilor coordinativi ai cuprului(II) cu tiosemicarbazone (Cu-CCT) asupra markerilor stresului oxidativ i a sistemelor antioxidante la nivel eritrocitar.S-au utilizat 120 de obolani (Rattus norvegicus albinus) care au fost mprii n 10 grupe, subdivizate ulterior n funcie de sex.Grupul de control a primit soluie salin, n timp ce celelalte grupuri au primit subcutanat 10 g/kg Cu-CCT timp de 30 de zile.Cu-CCT au generat specii reactive de oxigen (ROS) i au stimulat mecanismele antioxidante.Astfel, s-au determinat modificrile nivelurilor de dialdehid malonic (MDA), catalaz (CAT), superoxid dismutaz (SOD) i enzime implicate n metabolismul glutationului.Adiional, s-a observat o posibil influen asupra reglrii creterii celulare i a apoptozei, intermediat de modificrile statutului oxidant/antioxidant.Cu-CCT au un efect dual, crescnd anumii markeri pro-oxidani i, concomitent, amplificnd rspunsurile antioxidante, sugernd o potenial relevan terapeutic n afeciuni asociate cu stresul oxidativ.