THE INFLUENCE OF MORINGA OLEIFERA OIL ON NEUROBLASTOMA BASAL OXIDATIVE STRESS, INFLAMMATION AND AUTOPHAGY MECHANISM

Despite significant medical advances in neuroblastoma (NB) treatment, therapy resistance is a significant barrier to access to curative cancer treatments.This study investigates the effects of different concentrations of M. oleifera oil on drug resistance and reaction, basal oxidative stress in the SH-SY5Y neuroblastoma cell line, on one hand, and autophagy as a mechanism for tumour and cell survival on the other.First, we profiled the fatty acid composition of seed oils from M. oleifera using gas chromatography-mass spectrometry (GC-MS).Using different concentrations of Moringa olivera oil (50 -200 g/mL), the MTT cell proliferation assay was employed to assess cell survival in SH-SY5Y cell lines.Specific antioxidants and proinflammatory markers were estimated; LC3 as a specific marker for autophagy was evaluated, using a flow cytometer.LC3 expression was significantly higher in the SH-SY5Y-treated cell line at the highest concentration (200 g/mL) compared to the control.A status of dose and time independent reductions in pro-inflammatory cytokines and free radicals as markers of neuroinflammation and oxidative stress were recorded.M. oleifera oil could be used to reduce SH-SY5Y drug resistance initially by antioxidant and anti-inflammatory properties, rather than the essential role of autophagy, which is a novel finding as it shifts focus from autophagy as the central mechanism of action. RezumatDei s-au realizat progrese semnificative n tratamentul neuroblastomului, rezistena la terapie rmne un obstacol major pentru dezvoltarea unor tratamente eficiente.Acest studiu a investigat efectele diferitelor concentraii de ulei de Moringa oleifera asupra rezistenei la medicamente, a stresului oxidativ bazal i a autofagiei ca mecanism de supravieuire tumoral i celular.S-a analizat compoziia n acizi grai a uleiurilor din seminele de M. oleifera utiliznd cromatografia de gaze cuplat cu spectrometrie de mas (GC-MS).Ulterior, utiliznd concentraii diferite de ulei de M. oleifera (50 -200 g/mL), a fost evaluat supravieuirea celular n linia SH-SY5Y prin testul MTT de proliferare celular.De asemenea, au fost determinate nivelurile unor markeri specifici ai stresului oxidativ i inflamaiei, iar expresia LC3, marker al autofagiei, a fost analizat prin citometrie n flux.Rezultatele au artat o cretere semnificativ a expresiei LC3 n celulele tratate cu concentraia maxim (200 g/mL) comparativ cu grupul martor.n plus, s-au observat reduceri ale citokinelor proinflamatorii i ale radicalilor liberi, markeri ai neuroinflamaiei i stresului oxidativ, ntr-un mod independent de doz i timp.Astfel, uleiul de M. oleifera ar putea reduce iniial rezistena la medicamente n linia celular SH-SY5Y prin proprietile sale antioxidante i antiinflamatorii, mai mult dect prin autofagie.