PURSLANE (PORTULACA OLERACEA L.) GROWING IN SOUTH KAZAKHSTAN – PHYTOCHEMICAL COMPOSITION OF ULTRASONIC HYDROETHANOLIC EXTRACT AND ITS PHARMACOLOGICAL ACTIVITY

Aerial parts of Portulaca oleracea L. growing in South Kazakhstan were subjected to ultrasonic extraction with 70% ethanol.The phytochemical composition of the extract was studied using the RP-HPLC/PDA and HPLC-ESI-QTOF-MS methods.The following extract activities were studied in vivo after its oral administration: acute toxicity in outbred white mice, as well as analgesic activity by the formalin test method and anti-inflammatory effect by the carrageenan oedema method in outbred white rats.A total of 19 compounds were identified in the extract by HPLC-ESI-QTOF-MS fingerprinting, mainly organic acids and their derivatives, and phenolic acid derivatives.According to RP-HPLC/PDA data, the main compounds of this extract were derivatives of ferulic and protocatechuic acids (2.502 mg/100 g d. m.).No signs of extract toxicity and mortality were observed, even at a dose of 2000 mg d. m./kg.The extract at doses of 100 mg d. m./kg and 200 mg d. m./kg exerted a significant reduction in the number of pain reactions in both early and late phases, as well as a marked anti-inflammatory effect.These in vivo data demonstrated, for the first time, an analgesic and anti-inflammatory effect following oral administration of ultrasonic hydroethanolic extract from Kazakh species of purslane. RezumatPrile aeriene ale plantei Portulaca oleracea L., cultivat n sudul Kazahstanului, au fost supuse unui proces de extracie ultrasonic cu etanol 70%.Compoziia fitochimic a extractului a fost analizat utiliznd metodele RP-HPLC/PDA i HPLC-ESI-QTOF-MS.Activitatea farmacologic a extractului a fost evaluat in vivo, dup administrare oral, prin determinarea toxicitii acute la oareci albi (linie heterogen), a activitii analgezice prin testul cu formalin i a efectului antiinflamator prin metoda edemului indus cu caragenan la obolani.Prin analiza HPLC-ESI-QTOF-MS, au fost identificai 19 compui n extract, predominant acizi organici i derivaii acestora, precum i derivai ai acizilor fenolici.Conform datelor obinute prin RP-HPLC/PDA, compuii principali din extract au fost reprezentai de derivaii acizilor ferulic i protocatechuic (2,502 mg/100 g substan uscat).Administrarea extractului n doz de pn la 2000 mg/kg substan uscat nu a fost asociat cu toxicitate sau mortalitate.Administrarea extractului n doze de 100 mg/kg i 200 mg/kg a determinat o reducere semnificativ a reaciilor dureroase att n faza timpurie, ct i n faza tardiv a testului cu formalin, precum i un efect antiinflamator pronunat.Aceste date in vivo demonstreaz, pentru prima dat, efectele analgezice i antiinflamatoare ale extractului hidroetanolic obinut prin ultrasunete dintr-o specie de Portulaca oleracea cultivat n Kazahstan.