Enzyme inhibition-enabled CRISPR/Cas12a biosensing system for heparin-related non-nucleic acid biomarkers
In contrast to conventional CRISPR/Cas12a systems, which rely on complex functional nucleic acids, protein switches, or allosteric transcription factor (aTF)-based signal conversion for non-nucleic acid analysis, this work achieves more facile quantification of non-nucleic acid biomarkers through a novel heparin-mediated Cas12a inhibition mechanism.
Authors
- Wenjiao Fan
- Xinrui Fei
- Renyi Ma (ORCID: https://orcid.org/0000-0002-6152-9050)
- Chenghui Liu (ORCID: https://orcid.org/0000-0001-8375-0242)
- Yueran Wang (ORCID: https://orcid.org/0000-0002-1420-4480)
Institutions
- Ministry of Education of the People's Republic of China (CN)
- Northwest University (CN)
- Shaanxi Normal University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc03376d
- Citations
- 1
- Primary Topic
- Heparin-Induced Thrombocytopenia and Thrombosis
- Type
- article
- Field-Weighted Citation Impact
- 2.01