Investigation of radioprotective effects of WR-1065 and acetylsalicylic acid by micronucleus assay

Background:The current study aimed to investigate the ability of acetylsalicylic acid (ASA) to prevent ionizing radiation-induced DNA damage in human lymphocytes via the micronucleus (MN) assay and compare it with the efficacy of the agent WR-1065 (2-[(3-Aminopropyl) amino] ethanethiol dihydrochloride), which exhibits radioprotective activity.Materials and Methods: Peripheral blood samples were obtained from four participants, and nine experimental groups were established.Blood samples were treated with 25 g/mL ASA and 40 g/mL WR-1065 for 30 min before irradiation.After treatment, the samples were irradiated with 6 MV X-rays in the linear accelerator (LINAC) unit at doses of 2 Gy and 4 Gy.All blood samples were then cultured for the micronucleus assay.Results: In vitro treatments with ASA and WR-1065 at the indicated doses did not result in statistically significant changes in MN frequencies (p0.05).Conclusion: Both drugs were did not exhibit radioprotective effects in the experimental model used.