Sequence Variation and In Silico Protein Characterization of γ-TMT Gene in Mutant Rodent Tuber (Typhonium flagelliforme Lodd.)

γ-tocopherol is an important antioxidant compound associated with anticancer activity in several plants. This study aimed to analyze the <i>γ-TMT</i> (<i>γ-tocopherol methyltransferase</i>) gene sequence and predict its protein structure in mutant rodent tuber (<i>Typhonium flagelliforme</i> Lodd.) plants. Degenerate primers were designed from homologous sequences in monocot species and used to amplify the <i>γ-TMT</i> gene. Amplification of the <i>γ-TMT</i> gene was observedin the mutant and the wild-type plants. The amplified region partially covers the <i>γ-TMT</i> gene, which has undergone mutations due to a combination of somaclonal variation and gamma irradiation. Sequence analysis revealed notable variations between mutant and wild-type lines, including base substitutions and deletions. Predicted protein structures based on the coding DNA sequence (CDS) revealed notable differences in helix and loop orientation, particularly in the C-terminal domain and central regions of the protein. These structural differences suggest potential links to increased tocopherol biosynthesis or biological activity; however, further experimental validation is required to confirm these functional implications. This study provides foundational insights into the link between the expression of the <i>γ-TMT</i> gene and tocopherol biosynthesis and supports the development of specific molecular markers in <i>T. flagelliforme</i>.