Interfacially self-assembled ChOx/HRP proteinosomes for rapid and sensitive cholesterol detection

This study developed a novel cholesterol biosensing system based on interfacial self-assembled ChOx/HRP dual-enzyme proteinosomes, eliminating the need for complex synthesis. The immobilization of ChOx and HRP on the proteinosome surface enhanced enzyme stability, while the confined spatial proximity of the enzymes significantly improved cascade reaction efficiency, enabling rapid and highly sensitive cholesterol detection.