Development and evaluation of a triplex real-time PCR assay for enhanced plague diagnostics in Madagascar

The developed triplex assay to molecularly diagnose Y. pestis in human samples improves the standard already in place and allows to resolve ambiguities previously associated with inconclusive results from duplex qPCR tests, thereby reinforcing the reliability and accuracy of this new technique. Implementing this new method into routine will enable a faster, more effective response to plague outbreaks by reducing the time needed to confirm plague cases and limiting the spread of the diseases. This new technique is also flexible and can be undertaken close to human cases with adequate biosecurity and biosafety measures.

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Publication Details

Journal
PLoS neglected tropical diseases
Published
2025-07-24
DOI
https://doi.org/10.1371/journal.pntd.0013278
Citations
2
Primary Topic
Yersinia bacterium, plague, ectoparasites research
Type
article
Field-Weighted Citation Impact
3.63
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article

Development and evaluation of a triplex real-time PCR assay for enhanced plague diagnostics in Madagascar

Anne‐Sophie Le Guern, Minoarisoa Rajerison, Guillain Mikaty, Beza Ramasindrazana et al.
2 citations
PLoS neglected tropical diseases
Yersinia bacterium, plague, ectoparasites research
3.63
article

Development and evaluation of a triplex real-time PCR assay for enhanced plague diagnostics in Madagascar

Anne‐Sophie Le Guern, Minoarisoa Rajerison, Guillain Mikaty, Beza Ramasindrazana, A. Rasamindrakotroka, Philippe Gauthier, Mamy Gabriel Randriamanantsoa, Soloandry Rahajandraibe, Jean-Claude Manuguerra, Lanto Andrianarijaona Maminirina, Zaïna I. Bodoarison, Zoé A. Rahalison, Gilbert Kayoko
article en
2 citations

Abstract

The developed triplex assay to molecularly diagnose Y. pestis in human samples improves the standard already in place and allows to resolve ambiguities previously associated with inconclusive results from duplex qPCR tests, thereby reinforcing the reliability and accuracy of this new technique. Implementing this new method into routine will enable a faster, more effective response to plague outbreaks by reducing the time needed to confirm plague cases and limiting the spread of the diseases. This new technique is also flexible and can be undertaken close to human cases with adequate biosecurity and biosafety measures.

PLoS neglected tropical diseasesVol. 19(7)
World Health Organization - Pakistan (PK), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR), University of Antananarivo (MG), Institut Pasteur (FR), Université de Montpellier (FR), Université Paris Cité (FR), Institut Agro Montpellier (FR), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), National Application Center for Pharmaceutical Research (MG), Institut Pasteur de Madagascar (MG), Centre de Biologie pour la Gestion des Populations (FR), Institut de Recherche pour le Développement (FR)
Good health and well-being
Openalex Percentile: Top 7%
Yersinia bacterium, plague, ectoparasites research
3.63
DOIOpen Access PDFOpenAlex
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