Protocol for direct reprogramming of canine fibroblasts to induced motor neurons
Direct reprogramming of canine fibroblasts to induced motor neurons in vitro opens the door to molecular characterization, functional analysis, and therapeutic screening of canine age-related neurodegenerative diseases. Here, we present a protocol for generating canine induced motor neurons directly from primary dermal fibroblasts. We provide a step-by-step guide for isolating and maintaining primary dermal fibroblasts from dogs, obtaining a stock of reprogrammable cells, and performing the direct reprogramming process. We then detail procedures for validating the motor neuron identity.
Authors
- Ana Paula Corona (ORCID: https://orcid.org/0000-0002-0032-7906)
- Ryan Anderson (ORCID: https://orcid.org/0000-0002-6095-694X)
- Susannah J. Sample (ORCID: https://orcid.org/0000-0001-8551-7460)
- Masatoshi Suzuki (ORCID: https://orcid.org/0000-0003-0194-8795)
Institutions
- University of Wisconsin–Madison (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-07-24
- DOI
- https://doi.org/10.1016/j.xpro.2025.103975
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Waisman Center
- University of Wisconsin Foundation
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Institute of Child Health and Human Development