Haemo and Biochemical Changes in the Evaluation of Antimalarial Drugs as Therapeutic Alternatives to Mainstream Trypanosides in Experimentally Induced Trypanosomosis in Wistar Rats

This study evaluated the haematological and biochemical effects of antimalarial drugs as therapeutic alternatives to conventional trypanocides in Wistar rats experimentally infected with Trypanosoma brucei brucei, T. congolense, and T. vivax. Although parasite suppression and clinical signs were recorded, they are not included in this manuscript. Fifty-four adult rats were randomly assigned into three experimental models: (A) infected then treated, (B) treated before infection, and (C) infected but untreated. Each model consisted of six groups, with three replicates per group, each comprising three rats, adhering to the 3Rs principle. Rats were inoculated intraperitoneally with 5 × 10⁴ trypanosomes/mL and sampled at 60, 120, and 180 minutes post-infection.