Evaluation of oocyst detection and PCR identification of chicken <i>Eimeria</i> species using intestinal contents of the rectum and cecum as different gastrointestinal tracts
In the present study, intestinal specimens were collected from layer chickens at a poultry slaughterhouse and used to evaluate infection with Eimeria species. Intestinal contents were extracted separately from the rectum (from the duodenum to rectum) and cecum and used for analyses. Using flotation detection methods, the number of eimerian oocysts per gram was higher in the cecum than rectum. Polymerase chain reaction analysis also detected a greater abundance of Eimeria species in the cecum than the rectum. These results suggest that analysis of cecal contents could be more useful for detecting and identifying Eimeria species.
Authors
- April Hari Wardhana (ORCID: https://orcid.org/0000-0003-0750-8467)
- Kazumi Sasai (ORCID: https://orcid.org/0000-0003-1469-6646)
- Hiromitsu Katoh
- Carlos K. Furuya (ORCID: https://orcid.org/0000-0002-6512-5029)
- Toshimitsu Hatabu (ORCID: https://orcid.org/0000-0002-9145-4758)
- Dyah Hayuningtyas Sawitri
- Makoto Matsubayashi (ORCID: https://orcid.org/0000-0003-3692-8946)
- Daikichi Morinaga
- Aruto Takano
Institutions
- University of the Philippines Los Baños (PH)
- Okayama University of Science (JP)
- Okayama University (JP)
- CTI Engineering (Japan) (JP)
- Osaka Metropolitan University (JP)
- National Research and Innovation Agency (ID)
Publication Details
- Journal
- Journal of Veterinary Medical Science
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1292/jvms.25-0252
- Primary Topic
- Coccidia and coccidiosis research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Forskningsrådet om Hälsa, Arbetsliv och Välfärd
- Japan Society for the Promotion of Science