Culture conditions of symbiotic fungus <i>Coprinellus radians</i> and its effects on seedlings of <i>Cremastra appendiculata</i> (D.Don.) Makino (Orchidaceae)

This study screened and optimized the formulations and culture conditions of strain at all levels of Coprinellus radians; building on this foundation, the symbiotic seedling cultivation of Coprinellus radians with Cremastra appendiculata was optimized to explore the best conditions. The results revealed that the suitable conditions for the cultivation of the parental strain of Coprinellus radians were PDA as the basal medium with the addition of 10 g·L −1 fructose, 5·g·L −1 yeast extract, and 15 g·L −1 bran, and the colony diameter was 83.90 ± 0.82 mm and the dry weight of the hyphae was 2.4 ± 0.1 mg under these conditions. Further studies show that the colony diameter of the parental strain of Coprinellus radians was 84.77 ± 1.42 mm and the dry weight of mycelium was 2.5 ± 0.1 mg at 35 °C, pH = 6.0, and dark incubation. Subsequently, the formulation suitable for the cultivation of the primary and cultivation strain was a mixture of willow wood, cottonseed hulls, and sweet potato flour in a mass ratio of 8:1:1 with a water content of 30%. The symbiotic seedling cultivation methods were feasible, the suitable conditions for the symbiotic cultivation of Cremastra appendiculata with Coprinellus radians were: 5 kg·m −2 of wood block dosage, 50 mg·m −2 of seed dosage, and 0.5 kg·m −2 of cultivation strain dosage, under which the germination rate of Cremastra appendiculata was 33.69 ± 1.71%, and the emergence rate was 2.81 ± 0.36%. Results of these studies have screened suitable culture conditions for the strain of Coprinellus radians and conducted preliminary experiments for the establishment of symbiotic seedling propagation technology for Cremastra appendiculata.