Beyond the known cuts: trypsin specificity in native proteins
Above-filter digestion proteomics (AFDIP) was applied to quantify trypsin cleavage preferences in native HeLa cell lysates. Lysine sites were cleaved faster than arginine ones, with cleavage rates modulated by the peptide's size and isoelectric point. These trends, absent in denatured proteomes, highlight trypsin's context-dependent behavior and inform protein engineering for optimal digestibility.
Authors
- Amir Ata Saei (ORCID: https://orcid.org/0000-0002-2639-6328)
- Roman A. Zubarev (ORCID: https://orcid.org/0000-0001-9839-2089)
- João M. Gaspar (ORCID: https://orcid.org/0000-0002-2678-0725)
- José C. Marques (ORCID: https://orcid.org/0000-0002-4832-4341)
- Bohdana Sokolova (ORCID: https://orcid.org/0000-0003-3751-7129)
Institutions
- Peoples' Friendship University of Russia (RU)
- Sechenov University (RU)
- Karolinska Institutet (SE)
- Universidade da Madeira (PT)
- University of Aveiro (PT)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc02378e
- Citations
- 2
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.51