Distinguishing isomeric <i>N</i>-methylhistidine peptidoforms by ion mobility mass spectrometry
Histidine residues in proteins undergo histidine methyltransferase-catalysed Nτ-His and Nπ-His methylation. Here, we demonstrate that ion mobility spectrometry enables separation and identification of peptides possessing the isomeric Nτ-methyl-His and Nπ-methyl-His residues, leading to the assignment of the naturally occurring methyl-His isomer.
Authors
- Janina Kamińska
- Jasmin Mecinović (ORCID: https://orcid.org/0000-0002-5559-3822)
- Nurgül Bilgin (ORCID: https://orcid.org/0000-0002-7097-7218)
- Ole N. Jensen (ORCID: https://orcid.org/0000-0003-1862-8528)
- Tina Ravnsborg (ORCID: https://orcid.org/0000-0002-6376-621X)
- Jakub Drożak (ORCID: https://orcid.org/0000-0002-3601-3845)
- Francis Berthias (ORCID: https://orcid.org/0000-0001-5178-8967)
- Paulina Emmel
- Shah Adil Ishtiyaq Ahmad (ORCID: https://orcid.org/0009-0003-1563-1032)
Institutions
- University of Southern Denmark (DK)
- University of Warsaw (PL)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc03712c
- Citations
- 3
- Primary Topic
- Mass Spectrometry Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 2.27