A Novel Combo Rapid Test Cassette: Accurate Detection of CPV, CCV and Giardia Lamblia in Canine Feces and Vomit

Canine parvovirus (CPV), canine coronavirus (CCV), and Giardia lamblia pose significant health risks to dogs, especially puppies, by causing severe gastrointestinal diseases. Traditional detection methods, such as microscopy and ELISAs, have limitations, including low sensitivity, specificity, and time consumption. To address these challenges, the PV + CCV + Giardia Lamblia Antigen Combo Rapid Test Cassette was developed as a comprehensive and efficient diagnostic tool. This study evaluated its accuracy and effectiveness compared to conventional methods, using specimens collected from veterinary hospitals. Results indicated high sensitivity (95.74% for CPV, 93.10% for CCV, and 95.12% for Giardia lamblia) and specificity (100.00% for CPV, 97.52% for CCV, and 95.87% for Giardia lamblia), with consistent 100% accuracy across different antigen concentrations. Cross-reactivity tests confirmed that common fecal substances did not interfere with results. While the test demonstrated reliability, further research should focus on validating its performance in varied canine populations and enhancing user-friendliness, ultimately improving canine disease management through accurate and prompt pathogen identification.