Virological Tools Fail to Properly Identify “Unusual” Hepatitis C Virus Subtypes Resistant to Direct-Acting Antiviral Drugs in The Gambia
We evaluated the ability of the commercial Sentosa SQ Hepatitis C Virus Genotyping Assay to identify the hepatitis C virus (HCV) genotype subtype in patients from The Gambia. Subtype was determined from the Sentosa-generated NS5B sequences using 3 bioanalytical methods: Sentosa SQ HCV Genotyping Assay bioanalytical tool, Geno2pheno, and the National Reference Center in-house method. The Sentosa assay result agreed with the reference method in only 2 of 13 cases. This study highlights the difficulty of correctly identifying HCV subtypes in a region of Africa with a high diversity of HCV genotype 1 and 2 subtypes that may be resistant to antivirals.
Authors
- Sainabou Drammeh
- Maud Lemoine (ORCID: https://orcid.org/0000-0002-9011-2991)
- Isabelle Chemin (ORCID: https://orcid.org/0000-0002-7188-5489)
- Jean–Michel Pawlotsky (ORCID: https://orcid.org/0000-0003-0745-7559)
- Mélissa N’Debi
- Stéphane Chevaliez (ORCID: https://orcid.org/0000-0001-6913-9017)
- Christophe Rodriguez (ORCID: https://orcid.org/0000-0002-9817-5006)
- Gibril Ndow (ORCID: https://orcid.org/0000-0001-5837-5110)
- Erwan Vo Quang (ORCID: https://orcid.org/0000-0002-8280-3426)
- Alexandre Soulier
- Vincent Leroy (ORCID: https://orcid.org/0000-0001-5372-4334)
- Arnaud Ly
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université Paris-Est Créteil (FR)
- Paris-Est Sup (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- MRC Unit the Gambia (GM)
- Centre de Recherche en Cancérologie de Lyon (FR)
- Hôpitaux Universitaires Henri-Mondor (FR)
- Institut Mondor de Recherche Biomédicale (FR)
- Imperial College London (GB)
Publication Details
- Journal
- The Journal of Infectious Diseases
- Published
- 2025-07-19
- DOI
- https://doi.org/10.1093/infdis/jiaf378
- Primary Topic
- Hepatitis C virus research
- Type
- article
- Field-Weighted Citation Impact
- 0.00