Bacteriological and molecular study of Staphylococcus aureus

In the United States, approximately 400,000 patients are infected with S. aureus in hospitals each year, and approximately 100,000 of these die from complications caused by the infections (Haque et al., 2018). S. aureus remains a deadly pathogen for humans, more than a century after it was identified. S.aureus infections continue to be associated with high morbidity and mortality, both in hospitals and in the community. A total of 200 fresh urine samples were collected from both male and female patients with UTI of different ages included in this study during February-June 2021. Of the sampled patients, 133 (66.5%) showed positive growth. No significant growth was observed in the remaining 67 patients (33.5%). Demographic information of patients who applied to AL-Hussein Teaching Hospital with symptoms of UTI, The current findings of this study showed that 133 (66.5%) isolates had positive growth of the pathogen collected from UTI patients, while 67 samples (33.5%) showed no significant growth. Statistically, there were no significant differences in bacterial growth distribution (P≤ 0.01). The distribution of patients according to gender and bacterial growth Thereupon, 85 suspected isolates, seen under the microscope as G(+) with the coke shape mainly grouped in clusters, compared to ten mannitol salt agar, were able to ferment mannitol and produced a yellow color due to acid production. They were catalase positive due to the production of the enzyme catalase, which distinguishes them from Streptococcus spp., and they were coagulase positive due to the production of the enzyme staphylothrombin.