Impact of L-Arginine and Licorice Extract Supplementation on Growth and Epiphyseal Cartilage Health in PCOS-Induced Rats

Polycystic Ovary Syndrome (PCOS) is a common endocrine disorder characterized by hyperandrogenism and ovarian dysfunction, often leading to metabolic and reproductive complications. Due to their antioxidant and anti-inflammatory properties, L-arginine and licorice extract have been explored for their potential in infertility management. This study examines the effects of L-arginine and licorice extract on body weight and epiphyseal cartilage morphology in a rat model of PCOS. Forty-eight female rats were divided into six groups (n = 8): Group I (Control) received no treatment; Group II (Licorice) was administered 150 mg/kg licorice extract for 21 days; Group III (L-Arginine) received 3.5 mg/kg L-arginine for 21 days; Group IV (PCOS Model) was induced using 1 mg/kg letrozole for 21 days; Group V (Licorice + PCOS) received licorice extract following PCOS induction; and Group VI (L-Arginine + PCOS) was treated with L-arginine after PCOS induction. The study assessed body weight, absolute weights of the genital system, uterus, and ovaries, along with the histological structure of epiphyseal cartilage. Both L-arginine and licorice extract significantly improved epiphyseal cartilage integrity and regulated body weight, suggesting their potential therapeutic role in PCOS management.