The effect of Wi-Fi on elastic and collagen fibres in the blood vessel wall of the chorioallantoic membrane

In this study we observed the effect of Wi-Fi on various fibrous components that form the wall of large blood vessels in the chorioallantoic membrane (CAM) of the chicken embryo. Chicken embryos in the experimental groups (Wi-Fi) were continuously exposed (24 h per day) to Wi-Fi radiation at a frequency of 2.4 GHz and an average power density of 300 µW/m 2 for 9 and 14 embryonic days (ED). Subsequently, the CAM samples from the control (CO) and experimental (Wi-Fi) groups were histologically processed and evaluated. The samples stained with haematoxylin and eosin did not reveal any notable differences in the structure of large blood vessels between the CO and Wi-Fi groups. However, the use of special staining methods showed adverse effect of Wi-Fi on the fibrous elements within the blood vessel wall. The optical density (OD) of elastic fibres significantly decreased from 0.294 ± 0.025 (CO 9) to 0.197 ± 0.022 (Wi-Fi 9) at ED 9 and from 0.706 ± 0.028 (CO 14) to 0.271 ± 0.031 (Wi-Fi 14) at ED 14. On the other hand, at ED 9, the OD of collagen fibres exhibited a notable increase from 0.178 ± 0.023 (CO 9) to 0.334 ± 0.020 (Wi-Fi 9). However, at ED 14, there was a significant decline of collagen fibres from 0.418 ± 0.033 (CO 14) to 0.327 ± 0.031 (Wi-Fi 14). Our results support the hypothesis that Wi-Fi could affect the fibrous elements of the large vessel wall and may play a role in the development of different cardiovascular disorders.