LC-MS/MS-guided separation of guaiacolane-type sesquiterpenes with anti-inflammatory activities from <i>Chrysanthemum indicum</i>

Four previously undescribed compounds, including three guaiacane-type sesquiterpenoids (1-3) and one oleanane-type triterpenoid (4), along with seven known sesquiterpenoids, were isolated from the aerial parts of <i>Chrysanthemum indicum</i> using LC-MS/MS-guided fractionation. Structures were elucidated by IR, UV, HR-ESI-MS, and 1D/2D NMR analyses, and their absolute configurations were determined by ECD calculations. The anti-inflammatory activity was evaluated in LPS-stimulated RAW264.7 cells with NF-κB translocation by high-content imaging (HCI). Compound 2 reduced NF-κB translocation (IC<sub>50</sub> = 9.70 μM) without cytotoxicity at 20 μM. Compounds 8 and 9 showed potent activity (IC<sub>50</sub> = 2.04 and 1.21 μM, respectively) and no cytotoxicity at 6.25 μM.

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Publication Details

Journal
RSC Advances
Published
2025-01-01
DOI
https://doi.org/10.1039/d5ra03586d
Primary Topic
Phytochemistry and Biological Activities
Type
article
Field-Weighted Citation Impact
0.00

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article

LC-MS/MS-guided separation of guaiacolane-type sesquiterpenes with anti-inflammatory activities from Chrysanthemum indicum

Yunshuang Hu, Shiyun Huang, Qing Ma, Zhongqiu Liu et al.
RSC Advances
Phytochemistry and Biological Activities
article

LC-MS/MS-guided separation of guaiacolane-type sesquiterpenes with anti-inflammatory activities from Chrysanthemum indicum

Yunshuang Hu, Shiyun Huang, Qing Ma, Zhongqiu Liu, Min Wei, Xinyue Li, Yanyu Zhang, Hua Zhou, Yefeng Cai, Peng Wu, Limei Miu
article en

Abstract

Four previously undescribed compounds, including three guaiacane-type sesquiterpenoids (1-3) and one oleanane-type triterpenoid (4), along with seven known sesquiterpenoids, were isolated from the aerial parts of <i>Chrysanthemum indicum</i> using LC-MS/MS-guided fractionation. Structures were elucidated by IR, UV, HR-ESI-MS, and 1D/2D NMR analyses, and their absolute configurations were determined by ECD calculations. The anti-inflammatory activity was evaluated in LPS-stimulated RAW264.7 cells with NF-κB translocation by high-content imaging (HCI). Compound 2 reduced NF-κB translocation (IC<sub>50</sub> = 9.70 μM) without cytotoxicity at 20 μM. Compounds 8 and 9 showed potent activity (IC<sub>50</sub> = 2.04 and 1.21 μM, respectively) and no cytotoxicity at 6.25 μM.

RSC AdvancesVol. 15(32)
Guangzhou University of Chinese Medicine (CN), Henan University of Traditional Chinese Medicine (CN), China Resources (China) (CN), Key Laboratory of Guangdong Province (CN), Guangdong Provincial Hospital of Traditional Chinese Medicine (CN)
National Natural Science Foundation of China, Natural Science Foundation of Guangdong Province, Guangzhou University of Chinese Medicine, Guangzhou University, National Key Research and Development Program of China, Guangdong Special Support Plan
Openalex Percentile: Top 87%
Phytochemistry and Biological Activities
DOIOpen Access PDFOpenAlex
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