Inhibition of <i>KIAA0101</i> Expression Can Regulate Cell Proliferation and Apoptosis in Colon Cancer

<b><i>Background:</i></b> Colon cancer is a prevalent malignant tumor of digestive tract occurring in the colon. Colon cancer is the third leading cause of deaths in the world. Basic research on colon cancer is of great importance to patients with the disease. <b><i>Methods:</i></b> In this article, the authors observed the expression of <i>KIAA0101</i> in different types of cancers in public database (GEPIA2), found a gene network associated with <i>KIAA0101</i> in STRING database, and explored the correlation of these genes with <i>KIAA0101</i> at the expression level and the immune cells associated with the expression of <i>KIAA0101</i>. Then the authors detected the affection of <i>KIAA0101</i> to the proliferation and apoptosis of cancer cells and verified the consistency of <i>KIAA0101</i> expression in 31 tumor patient tissues using the database. <b><i>Results:</i></b> <i>KIAA0101</i> is differentially expressed in all kinds of tumor cells, and it shows high expression in colorectal cancer tissues. The authors found 10 genes related to <i>KIAA0101</i>, including <i>CDC20</i>, <i>TOP2A</i>, <i>CCNB2</i>, <i>CCNA2</i>, and so on, all of which show positive correlation with <i>KIAA0101</i> expression. In addition, the result of this study showed that <i>KIAA0101</i> expression had a positive correlation with the infiltration of Th1 and Th2 cells, with correlation coefficients of 0.371 and 0.627, respectively. <i>KIAA0101</i> gene shows high expression in both HCT15 and SW480 cell lines. Inhibition of <i>KIAA0101</i> expression can increase <i>caspase 3/7</i> activity in both HCT15 and SW480 cell lines, inhibit the proliferation ability of the two cell lines, and inhibit <i>Bcl-2</i> gene expression. <i>KIAA0101</i> also has tumor-suppressing effects in mice. The expression of <i>KIAA0101</i> gene also shows a significant increase in the tissues collected from 31 patients with tumor. <b><i>Conclusions:</i></b> Inhibition of <i>KIAA0101</i> expression can regulate cell proliferation and apoptosis in colorectal cancer.