An effective fluorescent sensor for lipopolysaccharide-induced H<sub>2</sub>S detection and imaging in inflammatory cells, zebrafish, and mouse blood samples
In this work, a new fluorescent sensor (NAHCP) for the recognition of lipopolysaccharide-induced H<sub>2</sub>S detection and imaging in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples was constructed. Based on the <i>o</i>-aldehyde group (<i>o</i>-CHO) auxiliary 2,4-dinitrophenyl ether (DNP) group sulfide cleavage, this sensor shows high sensitivity and selectivity towards H<sub>2</sub>S, which can realize fluorescence "turn-on" detection. This sensor also features a very high response speed (∼2 min) and a large Stokes shift (113 nm) toward H<sub>2</sub>S. Additionally, the excellent detection limit (LOD) for H<sub>2</sub>S is as low as 25.6 nM. Impressively, this <i>o</i>-CHO auxiliary DNP group sulfide cleavage ensemble is further successfully applied for H<sub>2</sub>S detection in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples.
Authors
- Meiling Pang
- Shishan Xue (ORCID: https://orcid.org/0000-0003-3072-191X)
- Zhenhui Kang (ORCID: https://orcid.org/0000-0001-6989-5840)
- Linjie Yuan (ORCID: https://orcid.org/0000-0002-5641-6684)
- Jun He
- Xinrong Zou (ORCID: https://orcid.org/0000-0002-8999-3242)
- Gulixiayida Wumaier
Institutions
- Central South University (CN)
- Mianyang Third People's Hospital (CN)
- Sichuan Mianyang 404 Hospital (CN)
- Dongfeng General Hospital (CN)
- Second Xiangya Hospital of Central South University (CN)
- Education Department of Hunan Province (CN)
- Chongqing Medical University (CN)
Publication Details
- Journal
- RSC Advances
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ra03905c
- Citations
- 1
- Primary Topic
- Sulfur Compounds in Biology
- Type
- article
- Field-Weighted Citation Impact
- 1.11
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hunan Province