Characteristics, Antibiotic and Phage Susceptibility of Staphylococci Causing Mastitis

The aim of the work is to isolate the pathogen based on the polyetiology of mastitis and the predominant role of staphylococci in the pathogenesis of the disease; to differentiate them by morphological, cultural and biochemical properties, and to study antibiotic and phage sensitivity. 13 strains of staphylococci were isolated and identified from milk samples. Among them, 8 St. aureus and 5 St. epidermidis field isolates. Their high sensitivity to doxycycline was revealed by the disk-diffusion method, the qualitative index of which was S. In addition, the polyresistance of some strains to azithromycin and streptomycin was determined to be 38.5-38.5%, and to amoxicillin to be 23.10%. In the process of research, the complete lability of all staphylococcal strains to phage (100%) was revealed. Only 7.7% of staphylococci were found to be resistant to septaphage. The results obtained demonstrate the high lytic activity of phages and the prospect of using them as a drug devoid of side effects for the treatment of animals suffering from mastitis. A significant advantage of bacteriophage over other drugs is that bacteria do not have the property of developing resistance to it.

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Journal
GEORGIAN SCIENTISTS
Published
2025-07-21
DOI
https://doi.org/10.52340/gs.2025.07.03.18
Primary Topic
Mycobacterium research and diagnosis
Type
article
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article

Characteristics, Antibiotic and Phage Susceptibility of Staphylococci Causing Mastitis

Merab Natidze, Manana Kereselidze, Rusudani Uridia, Ana Chulukhadze
GEORGIAN SCIENTISTS
Mycobacterium research and diagnosis
article

Characteristics, Antibiotic and Phage Susceptibility of Staphylococci Causing Mastitis

Merab Natidze, Manana Kereselidze, Rusudani Uridia, Ana Chulukhadze
article en

Abstract

The aim of the work is to isolate the pathogen based on the polyetiology of mastitis and the predominant role of staphylococci in the pathogenesis of the disease; to differentiate them by morphological, cultural and biochemical properties, and to study antibiotic and phage sensitivity. 13 strains of staphylococci were isolated and identified from milk samples. Among them, 8 St. aureus and 5 St. epidermidis field isolates. Their high sensitivity to doxycycline was revealed by the disk-diffusion method, the qualitative index of which was S. In addition, the polyresistance of some strains to azithromycin and streptomycin was determined to be 38.5-38.5%, and to amoxicillin to be 23.10%. In the process of research, the complete lability of all staphylococcal strains to phage (100%) was revealed. Only 7.7% of staphylococci were found to be resistant to septaphage. The results obtained demonstrate the high lytic activity of phages and the prospect of using them as a drug devoid of side effects for the treatment of animals suffering from mastitis. A significant advantage of bacteriophage over other drugs is that bacteria do not have the property of developing resistance to it.

GEORGIAN SCIENTISTS
Samtskhe-Javakheti State University (GE), EU Business School, Geneva (CH)
Zero hunger
Openalex Percentile: Top 75%
Mycobacterium research and diagnosis
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