Repurposing proteomic nanoLC-MS platforms for untargeted metabolomics: evaluating DIA and polarity switching performance in human plasma
The polarity switching DIA approach for metabolomics reduced sample consumption and analysis time, but led to fewer detected features and annotations compared to separate polarity runs. These findings support the use of unified nanoLC-MS platforms for integrated multi-omics analysis.
Authors
- Frederico Garcia Pinto (ORCID: https://orcid.org/0000-0003-3882-4657)
- Omer S. Alkhnbashi (ORCID: https://orcid.org/0000-0001-8088-590X)
- Nasna Nassir (ORCID: https://orcid.org/0000-0002-8883-9630)
- Mohammed J. Uddin (ORCID: https://orcid.org/0000-0001-6867-5803)
- Nesrin Mohamed (ORCID: https://orcid.org/0000-0001-9162-7180)
- Nelson C. Soares (ORCID: https://orcid.org/0000-0003-2331-8532)
- Alexander D. Giddey (ORCID: https://orcid.org/0000-0002-2034-8122)
- Munazza Murtaza (ORCID: https://orcid.org/0000-0001-9793-6865)
- Rauda S. B. Almarri
Institutions
- Genomatica (United States) (US)
- Universidade Federal de Viçosa (BR)
- Genome Canada (CA)
- University of Sharjah (AE)
- Mohammed Bin Rashid University of Medicine and Health Sciences (AE)
- Nova Medical (United States) (US)
- National Institute of Health Dr. Ricardo Jorge (PT)
- Universidade Nova de Lisboa (PT)
Publication Details
- Journal
- Expert Review of Proteomics
- Published
- 2025-07-21
- DOI
- https://doi.org/10.1080/14789450.2025.2537210
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Mohammed Bin Rashid University of Medicine and Health Sciences