Nitrogen limitation causes a seismic shift in redox state and phosphorylation of proteins implicated in carbon flux and lipidome remodeling in Rhodotorula toruloides
We conclude that lipid accumulation is largely a consequence of carbon rerouting and autophagy governed by changes to PTMs, and not increases in the abundance of enzymes involved in central carbon metabolism and fatty acid biosynthesis. Our systems-level approach sets the stage for acquiring multidimensional data sets for protein structural modeling and predicting the functional relevance of PTMs using Artificial Intelligence/Machine Learning (AI/ML). Coupled to those bioinformatics approaches, the putative PTM switches that we delineate will enable advanced metabolic engineering strategies to decouple lipid accumulation from nitrogen limitation.
Authors
- Josie Eder (ORCID: https://orcid.org/0000-0002-2876-0496)
- Rosalie Chu (ORCID: https://orcid.org/0000-0001-7428-7647)
- Austin Gluth (ORCID: https://orcid.org/0000-0003-3078-8081)
- Kent Bloodsworth (ORCID: https://orcid.org/0000-0002-7596-1565)
- Pavlo Bohutskyi (ORCID: https://orcid.org/0000-0002-0462-8132)
- Xiaolu Li (ORCID: https://orcid.org/0000-0002-9845-1584)
- Weijun Qian (ORCID: https://orcid.org/0000-0002-5393-2827)
- Bin Yang (ORCID: https://orcid.org/0000-0003-1686-8800)
- Jeffrey J. Czajka (ORCID: https://orcid.org/0000-0002-6912-0137)
- Tong Zhang (ORCID: https://orcid.org/0000-0003-1036-7060)
- Jennifer E. Kyle
Institutions
- Pacific Northwest National Laboratory (US)
- Joint BioEnergy Institute (US)
- Washington State University (US)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2025-07-21
- DOI
- https://doi.org/10.1186/s13068-025-02657-y
- Citations
- 7
- Primary Topic
- Mitochondrial Function and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 5.64
Funders
- Washington State University
- Pacific Northwest National Laboratory