Molecular Study of Cryptosporidium parvum Parasitic Strains Isolated from Some Mosul City Hospitals

The current study aimed to diagnose Cryptosporidium parvum and identify the extent of its prevalence in people suffering from diarrhea, especially children under five years, this is through microscopic diagnostic methods using modified MZN and gimsa stain and the use of molecular diagnostic technology, as the study showed the infection rate by (37.71) % (43) samples of examined stool samples by using microscopical diagnosed methods. In addition to the results of molecular examination that were carried out using the polymerase reaction technique for 25 stool samples out of (114) samples (25 positive samples and 89 negative samples) microscopically to confirm the accuracy of the diagnosis by microscopic examination, as it was noted that there were infections with the Cryptosporidium parasite by (21.92) and DNA sequencing was performed for samples diagnosed with the Cryptosporidium parasite to show new isolation for the first time in Nineveh Governorate and was registered in the National Center for Genetic Information NCBI with the serial number 757376.