Preparation and lyophilization of caprine platelet rich fibrin

Platelet Rich Fibrin is evolving as a vital biomaterial for wound management for its cocktail of growth factors. This study was taken to prepare Platelet Rich Fibrin (PRF) from caprine blood, lyophilize it and to study its macroscopic appearance and yield. Blood samples were collected from 20 goats which were centrifuged immediately to prepare the PRF clot. The PRF clot was then lyophilized to obtain lyophilized PRF (LyPRF). The fresh and lyophilized yield of PRF was standardized. The average yield of fresh PRF and LyPRF were 2522.23±169.58 and 184.40±15.19 /10ml of blood respectively. There was more than 90% reduction in the weight of PRF clot during lyophilization. Thus, this study validates a protocol for preparation, lyophilization and standardization of yield from caprine blood that could be used as a heterologous platelet product in regenerative medicine.