Gene Expression Associated with Comparative study between Lepidium sativum extract and hormonal treatment of ovarian inactivity in crossbreed cows.

This research aimed to study the effect of Lepidium sativum (LS) seed extract and to compare between it and GnRH in treating inactive ovaries in cows by gene expression, the study was conducted on 27 lactating anestrus crossbreed cows, aged between 3-5years in Al-Thahab Al-Abiad village / Abu-Ghriab/ Baghdad province during June 2020-July 2021. These animals were suffered from ovarian inactivity after 60-90 days postpartum. These dairy cows were randomly separated in to three equal groups. (9cows on each). The 1st group treated by injected IM with 0.021mg/animal GnRH (5ml), the 2nd group treated with Lepidium sativum 25mg/orally/ for 10days (250mg/animal), the 3rd group left without treatment. Reproductive parameters included animal response, period of response, services number /conceptions, rate of pregnancy and days open. The results revealed that animal response was 7(77.7%), 6(66.6%) and 3(33.3%) in the 1st, 2nd and 3rd group respectively with significantly increase (P<0.01) for the 1st (GnRH treated group) and 2nd (LS treated group) in compare with the 3rd group (control) , the duration of response recorded 6.36±1.24, 13.72±2.45 and 87.2±0.1in the three groups respectively recording superior significantly (P<0.01) for treatment groups (G1 , G2) when compare with the control group (G3) and for G1 compared with G2, Pregnancy rate was 85.7%, 83.3% and 66.6% and days open recorded 106.35±5.47, 114.26±6.52 and 187.40±0.0 in the 1st, 2nd and 3rd group respectively which was high significant in (P<0.01) in treatment groups in comparative with control group. We concluded that LS seed extract was effective in cows