Rapid visual detection of the severely destructive invasive pest <scp><i>Carpomya pardalina</i></scp>: a novel loop‐mediated isothermal amplification (<scp>LAMP</scp>) assay for efficient on‐site diagnostics
The detection can be completed within 75 min from DNA extraction, significantly enhancing the detection speed. Meanwhile, the visualization test demands simple equipment and is easy to operate, making it suitable for use at the port front line and under field working conditions. In this study, a set of rapid and accurate techniques was established based on the loop-mediated isothermal amplification (LAMP) technology for the detection of C. pardalina. © 2025 Society of Chemical Industry.
Authors
- Kai‐Yun Fu (ORCID: https://orcid.org/0000-0002-1309-9885)
- Jianyu Deng (ORCID: https://orcid.org/0000-0002-7137-6705)
- Wen‐Chao Guo (ORCID: https://orcid.org/0000-0002-3265-6309)
- Xiaojun Guo (ORCID: https://orcid.org/0000-0003-0139-3486)
- Qiong Rao (ORCID: https://orcid.org/0000-0003-1052-752X)
- Yang Yu (ORCID: https://orcid.org/0000-0002-9258-786X)
- Fei Xu (ORCID: https://orcid.org/0000-0002-2469-6183)
- Xinhua Ding (ORCID: https://orcid.org/0000-0002-6510-5992)
Institutions
- Zhejiang A & F University (CN)
- Xinjiang Academy of Agricultural Sciences (CN)
- ZheJiang Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-07-16
- DOI
- https://doi.org/10.1002/ps.70049
- Primary Topic
- Insect behavior and control techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Huazhong Agricultural University
- Yunnan Agricultural University