Ionic liquid-assisted seed genomic DNA extraction for advanced sequencing applications
This is the first known instance of a whole genome sequence generated from DNA extracted with ILs. These findings mark a significant milestone in establishing ILs as promising alternatives to conventional methods for seed DNA extraction, with potential utility in third generation (long-read) sequencing experiments.
Authors
- Gabriel Johnson (ORCID: https://orcid.org/0000-0001-7875-9285)
- Shashini De Silva (ORCID: https://orcid.org/0000-0001-6174-6728)
- Philip C. Bentz (ORCID: https://orcid.org/0000-0003-2232-7488)
- Morgan R. Gostel (ORCID: https://orcid.org/0000-0002-3169-627X)
- Jared L. Anderson (ORCID: https://orcid.org/0000-0001-6915-8752)
- Cecilia Cagliero (ORCID: https://orcid.org/0000-0003-3512-6124)
Institutions
- Smithsonian Institution (US)
- Botanical Research Institute of Texas (US)
- Iowa State University (US)
- Museum Conservation Institute (US)
- HudsonAlpha Institute for Biotechnology (US)
- University of Turin (IT)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-07-16
- DOI
- https://doi.org/10.1186/s13007-025-01417-1
- Citations
- 3
- Primary Topic
- Plant Disease Resistance and Genetics
- Type
- article
- Field-Weighted Citation Impact
- 5.25
Funders
- Iowa State University