Immunohistochemistry to Highlight Intraepithelial Lymphocytes in Formalin-Fixed and Frozen Duodenal Biopsies: Comparability of Results and Cut-Off Revision
Upon validation of an IHC protocol to stain TcRγδ+ IELs on FFPE duodenal sections that shows high sensitivity and specificity to discriminate CD patients from controls, this study establishes cut-off values for TcRγδ+ and CD3+ IELs on IHC-stained OCT and FFPE sections, showing highly comparable results for both embedding methods. Notably, this will widen the use of TcRγδ+ IELs density assessment in clinical practice, allowing a more precise diagnostic definition of difficult CD cases.
Authors
- Riccardo Troncone
- Maria Maglio (ORCID: https://orcid.org/0000-0002-9430-3779)
- Renata Auricchio (ORCID: https://orcid.org/0000-0001-6958-1251)
- A P Mirra (ORCID: https://orcid.org/0000-0001-6771-1788)
- Valentina Discepolo (ORCID: https://orcid.org/0000-0001-6158-0545)
- P F Angelino
- Antonella Marano
- Roberta Mandile (ORCID: https://orcid.org/0009-0008-1806-0147)
- Luigi Greco (ORCID: https://orcid.org/0000-0002-9443-5325)
- A D’Ambrosio (ORCID: https://orcid.org/0000-0002-2045-5155)
Institutions
- Federico II University Hospital (IT)
- University of Naples Federico II (IT)
Publication Details
- Journal
- Digestive Diseases and Sciences
- Published
- 2025-07-16
- DOI
- https://doi.org/10.1007/s10620-025-09168-8
- Citations
- 4
- Primary Topic
- Celiac Disease Research and Management
- Type
- article
- Field-Weighted Citation Impact
- 7.24
Funders
- European Commission