Optimization of Metagenomic Next-Generation Sequencing Workflow with a Novel Host Depletion Method for Enhanced Pathogen Detection
The workflow with the novel host depletion method significantly enhanced the analytical sensitivity of gDNA-based mNGS by reducing the host DNA background and enriching microbial content. This approach improved diagnostic yield in sepsis and may be a valuable tool for further clinical infectious disease diagnostics.
Authors
- Yen-Chia Chen (ORCID: https://orcid.org/0000-0003-2826-8503)
- Yen‐Wen Chen (ORCID: https://orcid.org/0000-0001-7974-1132)
- Chorng–Kuang How (ORCID: https://orcid.org/0000-0002-7419-0797)
- Chia-Ming Chang (ORCID: https://orcid.org/0000-0002-3059-6066)
- Po‐Hsiang Liao
- David Hung-Tsang Yen
Institutions
- National Yang Ming Chiao Tung University (TW)
- Taipei Veterans General Hospital (TW)
- National Defence Medical Centre (CA)
- National Defense Medical Center (TW)
Publication Details
- Journal
- Molecular Diagnosis & Therapy
- Published
- 2025-07-16
- DOI
- https://doi.org/10.1007/s40291-025-00797-3
- Citations
- 2
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Taipei Veterans General Hospital