Preparation of highly concentrated feline panleukopenia virus suspension

Feline panleukopenia control improvement requires preparation of relevant production and control virus strains and optimization of conditions for preparation of high-titre virus seed suitable for development of new vaccines and diagnostic test-systems. Optimized procedure for highly concentrated feline panleukopenia virus-containing suspension preparation is described in the paper. The following parameters were determined during the study: multiplicity of infection, time and conditions of the virus cultivation in continuous CRFK cell culture as well as the effect of cell confluency level and infection route on dynamics of reproduction of the virus isolate recovered in 2021. Besides, the virus-containing suspension purification and concentration procedures were optimized. As a result, purified feline panleukopenia virus suspension with high hemagglutinating activity (from 9,00±0,00 to 16,66±0,58 log2 HAU/0,1 cm3) was prepared. Data presented in the paper can be used for vaccine production and for development of schedules for laboratory animal hyperimmunization for diagnostic purposes.