Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis

Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Leishmania infection, harvesting infected tissues, and performing liquid chromatography-mass spectrometry (LC-MS) to identify enriched metabolic pathways and altered metabolites. We then detail procedures for verifying the metabolite role in immune response via PCR and ELISA. This protocol focuses on cutaneous leishmaniasis models, but the analyses here are applicable to other infection models. For complete details on the use and execution of this protocol, please refer to Volpedo et al.<sup>1</sup> and Oljuskin et al.<sup>2</sup>.

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Publication Details

Journal
STAR Protocols
Published
2025-07-16
DOI
https://doi.org/10.1016/j.xpro.2025.103817
Primary Topic
Research on Leishmaniasis Studies
Type
article
Field-Weighted Citation Impact
0.00

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article

Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis

Hira L. Nakhasi, Timur Oljuskin, Abhay R. Satoskar, Greta Volpedo et al.
1 links
STAR Protocols
Research on Leishmaniasis Studies
article

Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis

Hira L. Nakhasi, Timur Oljuskin, Abhay R. Satoskar, Greta Volpedo, Shinjiro Hamano, Laura Klenow, Matthew C. Bernier, Thalia Pacheco‐Fernández, Nazli Azodi, Greg Matlashewski, Sreenivas Gannavaram, Hannah L. Markle, Parna Bhattacharya
article en

Abstract

Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Leishmania infection, harvesting infected tissues, and performing liquid chromatography-mass spectrometry (LC-MS) to identify enriched metabolic pathways and altered metabolites. We then detail procedures for verifying the metabolite role in immune response via PCR and ELISA. This protocol focuses on cutaneous leishmaniasis models, but the analyses here are applicable to other infection models. For complete details on the use and execution of this protocol, please refer to Volpedo et al.<sup>1</sup> and Oljuskin et al.<sup>2</sup>.

STAR ProtocolsVol. 6(3)
Agricultural Research Service (US), Center for Biologics Evaluation and Research (US), Center for Devices and Radiological Health (US), Beltsville Agricultural Research Center (US), Nagasaki University (JP), McGill University (CA), The Ohio State University (US), University of Genoa (IT)
Global Health Innovative Technology Fund
Zero hunger
Openalex Percentile: Top 76%
Research on Leishmaniasis Studies
DOIOpen Access PDFOpenAlex
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Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis — Hira L. Nakhasi, Timur Oljuskin, et al. · STAR Protocols (2025) | TGRS Research Map | TGRS