Study on Joint Intensity in Real-Space and k-Space of SFS Super-Resolution Imaging via Multiplex Illumination Modulation

This paper studied the general mechanism of spatial-frequency-shift (SFS) super-resolution imaging based on multiplex illumination modulation. The theory of SFS joint intensity was first proposed. Experiments on parallel slots with discrete spatial frequency (SF) distribution and V-shape slots with continuous SF distribution were carried out, and their real-space images and k-space images were obtained. The influence of single illumination with different SFS and mixed illumination with various combinations on SFS super-resolution imaging was analyzed. The phenomena of sample SF coverage were discussed. The SFS super-resolution imaging characteristics based on low-coherence illumination and highly localized light fields were discovered. The phenomenon of image magnification during SFS super-resolution imaging process was discussed. The differences and connections between the SF spectrum of objects and the k-space images obtained in SFS super-resolution imaging process were explained. This provides certain support for optimization of high-throughput SFS super-resolution imaging.

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Publication Details

Journal
Photonics
Published
2025-07-16
DOI
https://doi.org/10.3390/photonics12070717
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
Field-Weighted Citation Impact
0.00

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article

Study on Joint Intensity in Real-Space and k-Space of SFS Super-Resolution Imaging via Multiplex Illumination Modulation

Feihong Lin, Xiaoyu Yang, Qing Yang, Xü Liu et al.
Photonics
Advanced Fluorescence Microscopy Techniques
article

Study on Joint Intensity in Real-Space and k-Space of SFS Super-Resolution Imaging via Multiplex Illumination Modulation

Feihong Lin, Xiaoyu Yang, Qing Yang, Xü Liu, Haonan Zhang
article en

Abstract

This paper studied the general mechanism of spatial-frequency-shift (SFS) super-resolution imaging based on multiplex illumination modulation. The theory of SFS joint intensity was first proposed. Experiments on parallel slots with discrete spatial frequency (SF) distribution and V-shape slots with continuous SF distribution were carried out, and their real-space images and k-space images were obtained. The influence of single illumination with different SFS and mixed illumination with various combinations on SFS super-resolution imaging was analyzed. The phenomena of sample SF coverage were discussed. The SFS super-resolution imaging characteristics based on low-coherence illumination and highly localized light fields were discovered. The phenomenon of image magnification during SFS super-resolution imaging process was discussed. The differences and connections between the SF spectrum of objects and the k-space images obtained in SFS super-resolution imaging process were explained. This provides certain support for optimization of high-throughput SFS super-resolution imaging.

PhotonicsVol. 12(7)
Zhejiang University of Science and Technology (CN), Zhejiang University (CN)
National Natural Science Foundation of China, National Key Research and Development Program of China
Openalex Percentile: Top 88%
Advanced Fluorescence Microscopy Techniques
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