Target of Rapamycin (TOR) signaling coordinates a balance between wheat photosynthetic performance and transpirational water conservation for improved water use efficiency and performance under drought

Drought is an important abiotic stress limiting wheat production worldwide. Hence there is a critical need to develop wheat varieties with improved performance under drought. Target of Rapamycin (TOR) kinase is a central regulator that integrates diverse nutrient, energy, hormone, and environmental stress response signals to coordinate plant growth and development. Recent studies have demonstrated that TOR is also involved in plant responses to abiotic stress. In this current study, in order to address TOR functions in response to wheat drought stress, we generated transgenic wheat lines expressing TaTOR under the control of constitutive and drought-inducible promoters. Inhibition of plant growth in response to drought was discovered to be closely associated with the expression and activity of the wheat TOR protein. Enhancing TaTOR expression driven by a constitutive promoter ( UBQ ) or drought-inducible promoters ( DREB/DEH ), significantly improved drought resistance and greatly reduced yield losses caused by drought stress in wheat. Examination of plant water relations, other related physiological parameters, and genome-wide transcriptomic comparisons demonstrated that enhancing TaTOR expression under drought helps wheat minimize transpirational water loss without compromising photosynthetic performance, thus improving water-use efficiency. This is achieved through efficient regulation of stomatal closure, along with enhanced photosynthetic efficiency, upregulation of ABA-mediated stress signaling, increased antioxidant capacity, and more robust recovery from drought. Our findings highlight the functional roles of TaTOR in wheat drought resistance, providing a valuable new molecular tool for developing wheat cultivars with improved drought resistance needed to address the drought and climate change challenges threatening wheat productivity worldwide. • TaTOR acts as a positive regulator of wheat resistance to drought stress. • Enhancing TaTOR expression under drought stress significantly improved wheat drought resistance, reducing yield losses under drought stress. • TaTOR expression under drought reduced transpirational water loss while maintaining photosynthetic performance, enhancing overall water-use efficiency. • TaTOR expression under drought facilitated efficient stomatal regulation and increased antioxidant capacity, contributing to better drought adaptation. • The function of TaTOR in wheat drought resistance highlights its potential as a valuable molecular tool for developing wheat cultivars with improved drought tolerance.