Development of Quantitative Analysis Method for Liriope platyphylla Extract Using Spicatoside A

In this study, we developed a reliable HPLC-UV (PDA) method for the quantification of spicatoside A in Liriope platyphylla extract. To overcome analytical interference, we introduced a solid-phase extraction (SPE) pretreatment using a C18 stationary phase to selectively remove hydrophilic components prior to analysis. Method validation was conducted in accordance with the guidelines of the Ministry of Food and Drug Safety (MFDS) of Korea, evaluating specificity, linearity, accuracy, precision (repeatability and reproducibility), and limit of quantitation. The developed method demonstrated high specificity, excellent linearity (R² > 0.9998), acceptable accuracy (92.8–105.2%), and precision within the criteria defined by the MFDS. The limit of quantitation for spicatoside A was determined to be 1.24 μg/ml. Although the pretreatment process is relatively labor-intensive, the method does not require expensive instruments such as HPLC-MS, offering advantages in both practicality and economic feasibility for small-scale research settings.