PIEZO1 mechanical insensitivity in generalized lymphatic dysplasia with the potential for pharmacological rescue
<i>PIEZO1</i> variants have been associated with generalized lymphatic dysplasia (GLD) through mechanisms involving reduced PIEZO1 expression. Here, we report variants where the mechanism involves reduced channel mechanical sensitivity. Two of the variants encode amino acid changes in the channel's cap structure (Ile2270Thr and Arg2335Gln), one in the ninth transmembrane helical unit (THU) below the cap (Gly1978Asp) and one in the fifth THU distant from the cap (Glu829Val). Patch-clamp studies of the cap and sub-cap variant channels revealed abolished or reduced channel mechanical sensitivity with the possibility to activate the channels and partly rescue mechanical sensitivity by the small molecule Yoda1. The potency of Yoda1 at the variant channels was less than at the wild-type channel, but chemical synthesis of Yoda1 analogs revealed a molecule with improved potency. The data suggest cases of GLD in which there is decreased channel mechanical sensitivity and the potential to reduce dysfunction pharmacologically.
Authors
- Elisavet Fotiou (ORCID: https://orcid.org/0000-0002-9789-2599)
- Richard Foster (ORCID: https://orcid.org/0000-0002-2361-3884)
- Natalie B. Tan (ORCID: https://orcid.org/0000-0003-3339-7342)
- Esther Dempsey (ORCID: https://orcid.org/0000-0002-7653-4653)
- Oleksandr V. Povstyan (ORCID: https://orcid.org/0000-0002-6998-3944)
- Claire Hogg (ORCID: https://orcid.org/0000-0002-8368-5994)
- Kevin Cuthbertson (ORCID: https://orcid.org/0000-0002-8344-287X)
- Antreas C. Kalli (ORCID: https://orcid.org/0000-0001-7156-9403)
- David J. Beech (ORCID: https://orcid.org/0000-0002-7683-9422)
- G Parsonage (ORCID: https://orcid.org/0000-0003-0648-4539)
- Sahar Mansour (ORCID: https://orcid.org/0000-0001-6629-4118)
- Tobias Linden (ORCID: https://orcid.org/0000-0003-3896-262X)
- Ege Sackey (ORCID: https://orcid.org/0000-0001-5477-0965)
- Andrew Bush (ORCID: https://orcid.org/0000-0001-6756-9822)
- Susan M. White (ORCID: https://orcid.org/0000-0001-7611-634X)
- Melanie J. Ludlow (ORCID: https://orcid.org/0000-0002-0720-5581)
- Charlotte Revill
- Emily Fay (ORCID: https://orcid.org/0000-0002-0133-8420)
- Silvia Martin‐Almedina (ORCID: https://orcid.org/0000-0001-5282-6662)
- Pia Østergaard (ORCID: https://orcid.org/0000-0002-2190-1356)
- Juan Carlos Del Rey Jimenez
- Deborah M Linley
- Katie A. Smith (ORCID: https://orcid.org/0009-0005-6664-2748)
Institutions
- University of Leeds (GB)
- Royal Brompton Hospital (GB)
- Klinikum Oldenburg (DE)
- Victorian Clinical Genetics Services (AU)
- Murdoch Children's Research Institute (AU)
- Imperial College London (GB)
Publication Details
- Journal
- iScience
- Published
- 2025-07-16
- DOI
- https://doi.org/10.1016/j.isci.2025.113110
- Citations
- 1
- Primary Topic
- Erythrocyte Function and Pathophysiology
- Type
- article
- Field-Weighted Citation Impact
- 1.16
Funders
- Wellcome
- Wellcome Trust
- Newlife the Charity for Disabled Children
- National Institute for Health and Care Research
- British Heart Foundation
- University of Leeds
- Medical Research Council
- NIHR Imperial Biomedical Research Centre
- Leeds Biomedical Research Centre