INVESTIGATION OF THE LEVEL TNF-ALPHA IN THE BLOOD SERUM OF GUINEA PIGS UNDER CONDITIONS OF EXPERIMENTAL ALLERGIC ALVEOLITIS ASSOCIATED WITH EXPERIMENTAL PNEUMONIA AND ITS CORRECTION WITH CORVITIN AND L-ARGININE

Introduction. TNP-α is produced by immunocompetent cells and is involved in the human body's complex regulation of inflammatory and immune processes. Because of this, it can be an important prognostic criterion for various diseases, including comorbid pathology, which has recently been given considerable attention in scientific publications.The purpose of the study. Determine the level of TNF-α in serum of guinea pigs under conditions of formation of experimental allergic alveolitis associated with experimental pneumonia and during correction with corvitis and L-arginine. To find out the role of these changes for the pathogenesis of the studied pathologies.Material and methods. Experimental studies on the determination of TNF-alpha were performed in the blood serum of guinea pigs (males) with experimental allergic alveolitis in combination with experimental pneumonia on days 7, 14, 21 of the experiment. The model of experimental allergic alveolitis (EAA) was reproduced according to Regeda M.S. The experimental pneumonia (EP) model was reproduced using the Regeda M.S. methods. The level of TNF-α as a result of correction with corvitin and L-arginine was determined on day 21 of the experiment. The content of TNF-α was determined by a solid-phase enzyme-linked immunosorbent assay (ELISA). A set of reagents for quantitative enzyme-linked immunosorbent assay of hi-corresponding cytokines (Diacalone, France) was used.Results of the study. As a result of the examination, a significant increase in the level of TNF-α in the serum of guinea pigs with EAA is combined with EP. The highest level was recorded for 21 days of the experiment. As a result of treatment with corvitin, and corvitin with L-arginine, they recorded a decrease in the level of TNP- α, especially during the combination of two specified agents.Conclusions. Determining the level of TNF-α during the examined comorbid pathology can be an important prognostic factor in the diagnosis and treatment of diseases of the pulmonary pathology, as well as the criterion for the course of the inflammatory process.The immunoregulatory and anti-inflammatory role of cavities and L-arginine in treating comorbid pathology, namely experimental allergic alveolitis combined with experimental pneumonia in guinea pigs, has been proven.